Figure EV3. Immortalized human cerebral microvascular endothelial cells (hCMEC/D3) transduced by brain‐targeted vectors.

Vector‐mediated eGFP expression under the control of the CAG promoter (green) and DAPI staining of nuclei (blue) are shown. AAV‐BR1‐eGFP (left panel), AAV2‐WT‐eGFP (middle panel), and AAV‐PPS‐eGFP (right panel), 4 days after infection with 1.6 × 10¹⁰ genomic particles of recombinant vector per well. The bar graph on the right shows infectivity, counted as GFP‐positive in eight randomly taken images from two wells per virus. Data are shown as bars (mean) with plotted individual data points.