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. 2016 May 3;8(6):670–678. doi: 10.15252/emmm.201505994

Figure 1. Loss of Ifnar1 signaling elicits inflammatory processes and angiogenesis in laser‐induced retinal damage.

Figure 1

  • A
    Experimental design. Laser coagulation was performed in C57BL6/J control and Ifnar1 −/− mice. Animals were analyzed 3, 7, and 14 days after laser treatment.
  • B, C
    Representative Iba1 stainings of retinal flat mounts detecting microglia/macrophages in laser spots 3 days after laser coagulation in C57BL6/J controls (B) and Ifnar1 −/− (C) mice. Scale bar: 20 μm.
  • D
    Quantification of amoeboid‐shaped mononuclear phagocytes in laser spots. Values show mean ± SD (n = 11–12 retinas; unpaired Student's t‐test: ***P = 0.0004).
  • E
    Quantification of immune cell morphology in laser spots using a grid image analysis system. Values show mean ± SD (n = 42–62 cells; unpaired Student's t‐test: ***P < 0.0001).
  • F–K
    Representative fundus fluorescein angiography images of C57BL6/J (F–H) and Ifnar1 −/− (I–K) mice 3, 7, and 14 days after laser‐induced damage.
  • L
    Quantification of vascular leakage by analyzing pixel intensities at 3, 7, and 14 days after laser‐induced retinal damage in C57BL6/J controls versus Ifnar1 −/− mice. Values show mean ± SD (n = 14–22 eyes; one‐way ANOVA followed by Tukey's post‐test: ***P < 0.0001).
  • M–P
    Representative images of lectin‐stained choroidal flat mounts 7 and 14 days after laser coagulation in C57BL6/J control mice (M, N) and Ifnar1 −/− animals (O, P). Dashed lines indicate CNV areas, and the asterisk marks the central optic nerve head. Scale bar: 200 μm.
  • Q
    Quantification of lectin‐stained CNV areas with ImageJ software. Bars show mean ± SD (n = 4–11 RPE/choroidal flat mounts; one‐way ANOVA followed by Tukey's post‐test: *P = 0.0281).