Table 1. Natural product extract hits by plate.
| Plate numbers | 1–8 | 9–15 | 16–23 | All plates |
|---|---|---|---|---|
| Collection dates | 2007–2009 | 2010–2011 | 2012–2014 | 2007–2014 |
| ISP2 media | ||||
| No. of extracts | 4663 | 3837 | 2371 | 10 871 |
| >50% inhibition | 1.5% | 10.2% | 37.1% | 12.3% |
| >90% inhibition | 0.4% | 1.7% | 3.5% | 1.6% |
| Nutrient poor media | ||||
| No. of extracts | 4625 | 3902 | 2281 | 10 808 |
| >50% inhibition | 0.5% | 4.1% | 13.8% | 4.6% |
| >90% inhibition | 0.2% | 0.6% | 0.9% | 0.5% |
| A3M Media | ||||
| No. of extracts | – | – | 1062 | 1062 |
| >50% inhibition | – | – | 49.1% | 49.1% |
| >90% inhibition | – | – | 8.9% | 8.9% |
| Corynebacterium Co-culture | ||||
| No. of extracts | – | – | 755 | 755 |
| >50% inhibition | – | – | 56.6% | 56.6% |
| >90% inhibition | – | – | 9.5% | 9.5% |
| Rhodococcus Co-culture | ||||
| No. of extracts | – | – | 1022 | 1022 |
| >50% inhibition | – | – | 46.3% | 46.3% |
| >90% inhibition | – | – | 8.0% | 8.0% |
| Other media | ||||
| No. of extracts | 689 | 958 | 2734 | 4381 |
| >50% inhibition | 34.0% | 16.2% | 21.0% | 22.0% |
| >90% inhibition | 9.6% | 3.5% | 3.3% | 4.3% |
| All media types | ||||
| No. of extracts | 9977 | 8697 | 10 225 | 28 899 |
| >50% inhibition | 3.3% | 8.1% | 31.2% | 14.6% |
| >90% inhibition | 0.9% | 1.4% | 4.3% | 2.3% |
| Extract selection | 96 | 336 | 895 | 1327 |
| Confirmation screen | ||||
| >50% inhibition (triplicate) | 20.8% | 38.4% | 55.6% | 48.8% |
| >90% inhibition (triplicate) | 8.3% | 10.4% | 12.5% | 11.7% |
| Luciferase counterscreen | ||||
| <50% inhibition (triplicate) | 89.6% | 65.8% | 71.2% | 71.1% |
| <20% inhibition (triplicate) | 40.6% | 42.6% | 28.4% | 32.9% |
| Final extract selection | 8 | 31 | 71 | 110 |