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Reoviruses that display the HIV-1 2F5 epitope in attachment protein σ1 replicate efficiently in cell culture. L cells were adsorbed with rsT1L, rsT1L σ1/2F5-56, or rsT1L σ1/2F5-147 at an MOI of 1 PFU/cell. Viral titers in cell lysates were determined by plaque assay at 0, 24, and 48 h postadsorption. Results are expressed as the mean viral yield for triplicate samples. Error bars indicate standard deviations. *, P < 0.05 compared with rsT1L (Student’s t test).
