FIG 3 .

The 2F5 epitope is displayed by reovirus vectors encoding single 2F5 substitutions. Wells of FLISA plates were coated with 10¹¹ particles of rsT1L, rsT3D, rsT1L σ1/2F5-56, or rsT1L σ1/2F5-147 and incubated with a T1L σ1 head-specific polyclonal antiserum (A) or MAb 2F5 (B) at 37°C for 1 h. Antibody binding to the immobilized virus was detected following incubation with fluorophore-conjugated donkey anti-human IgG. FLISA signals were quantified using a LI-COR Odyssey infrared imaging system.