FIG 7 .

Second-generation reovirus vectors display the MPER epitope. L cells plated in 96-well plates were adsorbed with 10-fold serial dilutions of rsT1L, rsT1L/σ1 MPER-63, or rsT1L/σ1 MPER-98 over a range of MOIs (0.01 to 100 PFU/cell). At 24 h postadsorption, cells were fixed and stained with 2F5 epitope-specific monoclonal antibody (A) or polyclonal reovirus-specific antiserum (B) followed by secondary antibodies conjugated to AlexaFluor 488. The results were quantified using an Odyssey infrared imaging system and normalized to the maximum response achieved for each antibody. The data are presented as percentages of the normalized response.