FIG 8 .

Immunogenicity of REO-MPER vectors. Six-week-old, reovirus-seronegative New Zealand White rabbits were inoculated perorally with 10⁹ PFU of T1L, rsT1L/σ1 MPER-63, or rsT1L/σ1 MPER-98 (n = 3 or 4 rabbits per group). Blood was collected on day 0 (preinoculation) and on days 14, 35, and 56 (postinoculation). Serial 4-fold dilutions of sera were tested for reovirus-specific antibodies by determining the PRNT60 (A) and HIV-specific antibodies by determining the capacity to neutralize infection of TZM-bl cells by tier 1 HIV-1 Env-pseudotyped viruses (B) or A3R5 cells by tier 2 HIV-1 Env-pseudotyped viruses (C). Results are expressed as the 50% infectious dose (ID₅₀) for pseudotyped viruses. Error bars indicate standard deviations.