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. 2016 May 19;1(7):e86873. doi: 10.1172/jci.insight.86873

Figure 1. Increased chemoresistance in murine mammary tumors lacking MCJ.

Figure 1

(A) MCJ expression in normal mammary gland from a WT female mouse (N, C57BL/6, The Jackson Laboratory) or in mammary tumors isolated from 2 independent MMTV-PyMT mice (T1, T2) examined by Western blot analysis using whole cell extracts. (B) Kaplan-Meier survival curve of MMTV-PyMT (MMTV) and MCJ KO MMTV-PyMT (MCJ KO MMTV) mice (n = 5). Death was determined as the time mice needed to be euthanized due to enlarged tumors. P > 0.05 by log-rank test. (C) Histology (H&E) of mammary tumors from MMTV and MCJ KO MMTV mice at ×200 magnification. Boxes indicate enlarged sections. (D) Tumors from MMTV and MCJ KO MMTV were harvested and dissociated. Tumor cells were plated and treated with doxorubicin for 16 hours. Mean ± SD of cell survival relative to cells without doxorubicin is shown (n = 3). (E) MMTV and MCJ KO MMTV (n = 4) mice were treated with doxorubicin by i.p. administration every other day for 2 weeks. The size of a tumor over time is represented as a percentage relative to the initial size prior to the treatment. *P < 0.05 by unpaired t test.