Skip to main content
. 2016 Apr 14;27(3):282–287. doi: 10.1097/MOL.0000000000000292

FIGURE 1.

FIGURE 1

Delivery of LDL-cholesterol to the plasma membrane and endoplasmic reticulum. Left: microscopic images of a cell protrusion showing the positioning of marker proteins Cherry-CD63 [highlighting late endosomes (LEs) and plasma membrane (PM)] and BFP-KDEL [highlighting endoplasmic reticulum (ER)]. Right: simplified schematic illustration of LDL-cholesterol delivery routes. Upon LDL endocytosis, LDL-cholesterol reaches multivesicular bodies (MVBs) and LEs. LDL-cholesterol can reach the PM via a Rab8a/Myo5b/actin-dependent vesicular trafficking pathway or potentially via membrane contact sites (MCSs) via other organelles, such as peroxisomes (P). PM cholesterol can be transported to the ER, potentially involving PM-ER MCSs. LDL-cholesterol can also reach the ER via vesicular trafficking, involving retrograde transport via the Golgi, or may undergo direct transport between LE and ER at MCSs. These MCSs remain to be molecularly characterized but may involve sterol binding proteins (see Table 1). BFP-KDEL was a gift from Gia Voeltz [44].