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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Mol Microbiol. 2015 Nov 17;99(4):627–639. doi: 10.1111/mmi.13259

Figure 2.

Figure 2

EIIAGlc (crr) activates CsrB/C decay via CsrD, but does not enhance cellular CsrD levels.

A and B. Decay rates of CsrB (A) and CsrC (B) were determined by Northern blotting of RNA from E. coli MG1655 (WT), ΔcsrD, Δcrr ΔcsrD strains with or without plasmid pBR322, pCRR or pBYH4 (expressing CsrD), as described in Figure 1.

C. Western blots depicting the effect of crr deletion on the level of CsrD protein. RpoB was used as loading control. CsrD protein levels in Δcrr relative to those in the wild-type strain (WT) are shown at the bottom. Standard derivations from triplicate experiments are indicated.