(A) PCR analysis to characterize transconjugant N315EX pRM27. Lane A, 1 kb molecular size marker; lanes 1 and 2, integrated attR and attL amplicons, respectively; lane 3, excised chromosomal junction (attB); lane 4, circular SCCmec junction (attS); lane 5, mecA; lane 6, orf0042, specific to RN4220; lane 7, ermA, specific to N315; lane 8, mupA; lane B, 500 bp molecular size marker
(B) PCR analysis to characterize transconjugant RN4220 pCN36 pRM27. The lanes are the same as in Fig. 3A.