Skip to main content
. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Mol Microbiol. 2016 Mar 14;100(4):675–685. doi: 10.1111/mmi.13340

Fig. 5.

Fig. 5

(A) pRM27 is a 64,127 bp plasmid consisting of the conjugative plasmid pGO400 (red) and captured, circularized SCCmec (blue). Loci of interest include the trs operon (purple) responsible for the conjugative transfer of the plasmid between strains and species; mupirocin resistance gene, mupA (green); IS elements (black), three from pGO400 (tnpC, tnpD, and tnpG) and two from SCCmec (tnpMec1 and tnpMec2). The insertion of SCCmec into tnpG and its duplication to form tnpMec2 are marked with red asterisks. The latter two flank pUB110 (grey) which also contains the gene for kanamycin resistance (aadD, light blue). Also shown are the mec operon (orange) and the ccr operon (pink) encoding CcrA and CcrB, the proteins responsible for site-specific recombination between attS (marked) and attB in the S. aureus genome. Accession number KT780705.

(B) Chromosomal map of the conjugative plasmid pGO400 showing the 3 IS431/IS257 elements in black, tnpG, the IS element into which SCCmec inserted is marked with an asterisk, mupirocin resistance gene mupA in green and the genes of the transferase (trs) operon in maroon. Accession number KT780704.