Table 1.
Filter matings and conjugation frequencies of pRM27. Conjugation frequencies are expressed as the number of transconjugants divided by the number of donors. Donor RN4220 pCN36 pRM27 was resistant to kanamycin, mupirocin, and tetracycline. Recipients were resistant to rifampin and novobiocin except for S. epidermidis VCU129 which was resistant to erythromycin. Donor S. epidermidis VCU129 pRM27 was resistant to kanamycin, mupirocin, and erythromycin. Sa, S. aureus; Se, S. epidermidis; CC, clonal complex; NC, not classified; ST, sequence type.
| Donor Strain | Recipient Strain | Recipient Species | CC(ST) | Donors CFU/ml | Recipients CFU/ml | Recipient + pRM27 transconjugants CFU/ml | Conjugation Frequency |
|---|---|---|---|---|---|---|---|
| RN4220 pCN36 pRM27 | Cowan I | Sa | 30(30) | 9.80E+09 | 8.40E+09 | 0 | 0 |
| RN4220 pCN36 pRM27 | 8325 | Sa | 8(8) | 1.74E+08 | 2.10E+08 | 2.00E+01 | 1.15E-07 |
| RN4220 pCN36 pRM27 | RN4220 | Sa | 8(8) | 1.13E+09 | 1.34E+09 | 3.80E+02 | 3.36E-07 |
| RN4220 pCN36 pRM27 | MR14EX | Sa | 5(5) | 1.04E+09 | 3.50E+09 | 1.10E+02 | 1.06E-07 |
| RN4220 pCN36 pRM27 | NRS242 | Sa | 121(121) | 1.23E+09 | 7.40E+08 | 0 | 0 |
| RN4220 pCN36 pRM27 | MW2EX | Sa | 1(1) | 1.02E+09 | 1.13E+09 | 6.00E+00 | 5.88E-09 |
| RN4220 pCN36 pRM27 | MSSA476 | Sa | 1(1) | 1.88E+09 | 1.02E+09 | 1.00E+00 | 5.32E-10 |
| RN4220 pCN36 pRM27 | 18-1-2 | Sa | 5(105) | 1.24E+09 | 1.18E+09 | 3.80E+01 | 3.06E-08 |
| RN4220 pCN36 pRM27 | VCU113 | Se | NC | 1.63E+08 | 7.40E+08 | 0 | 0 |
| RN4220 pCN36 pRM27 | VCU129 | Se | 193(193) | 1.03E+09 | 1.11E+09 | 8.40E+01 | 8.16E-08 |
| S. epidermidis VCU129 pRM27 | RN4220 | Sa | 8(8) | 2.30E+08 | 1.70E+08 | 4.50E+02 | 1.96E-06 |
| S. epidermidis VCU129 pRM27 | MR14EX | Sa | 5(5) | 2.10E+08 | 3.60E+08 | 6.00E+01 | 2.86E-07 |