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. 2016 Jun 1;11(6):e0155492. doi: 10.1371/journal.pone.0155492

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© 2016 Gilmore et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Cells stably expressing Halo-WDR76 were separately transiently transfected with CLIPtag™-FLAG proteins and HaloTag™ protein purifications conducted. Western blotting for tubulin was used as a loading control and western blotting with an anti-FLAG antibody was used to detect CBX1 (A), CBX3 (B), and CBX5 (C). Both the inputs and the results of the pulldown is shown. (D) Cells stably expressing Halo-WDR76 were transiently transfected with CLIPg™-FLAG construct to demonstrate that this construct was not responsible for the co-purifications of CBX proteins with WDR76.