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. 2016 Jun 1;11(6):e0156673. doi: 10.1371/journal.pone.0156673

Fig 4. GUS expression patterns (A-L) and RT-PCR (M) of PAP1pro:GUS (A-D, I-K) and PAP1wg:GUS (E-H, L) lines.

Fig 4

PAP1pro:GUS lines did not show expression in rosette (A) or cauline (B) leaves. Siliques usually showed patchy staining (D). No staining was observed in flower buds (C). Immature flowers (I, J) showed a band of staining just under the stigma, while mature flowers (C, K) showed expression in flower petals, ovaries, and filaments. PAP1wg:GUS lines showed staining in rosette (E) and cauline (F) leaves, flowers at all stages (G), and siliques (H). GUS staining was observed in sepals, ovaries, and filaments of flowers (L). Siliques of PAP1pro:GUS plants were not uniformly or consistently stained in this experiment. M. RT-PCR of 5 week old plants expressing PAP1 promoter and whole gene constructs. Scale bars are 3 mm in A, B, E, and F, 2 mm in C, D, G, and H, and 0.5 mm in I-L.