Figure 12.42.1.

Immuno-spin trapping of biomolecular free radicals in pulmonary tissue of obese mice. (Top) The reactions involved in the immuno-spin trapping (IST) process. (Bottom) A representative image of an airway and surrounding tissue that was generated by exposing a diet-induced obese mouse (C57Blk/6 J, subjected to a diet consisting of 60% calories derived from fat for 20 weeks) to 5,5-dimethyl-1-pyrroline N-oxide (DMPO). The presence of abundant DMPOadducted biomolecules (red) is seen in the pseudostratified ciliated columnar epithelial cells of the airway as well as the basolateral space, alveoli, and interstitium. This is indicative of elevated reactive species generation in these anatomic sites. This experiment was conducted with the approval of the University of Pittsburgh IACUC (protocol # 1009934 A). Red, DMPO; Blue, DAPI; Green, actin; 2°, secondary antibody.