Fig 1. Comparison of LPS binding in indirect ELISA with different buffers and blocking agents.

FITC-LPS from E. coli serotype O111:B4 was incubated at 1.25 μg/ml in PBS-EDTA (PBS), with or without 0.05% Tween 20 (PBS-TW2) or Triton X-100 (PBS-TTX), in wells coated with polymyxin B (PMX) or lysozyme (LSZ) (1 μg/well), or empty wells (control), which were previously blocked with BSA (A) or OVA (B). Each assay was performed in triplicate. Mean values ± SD are shown in the graph.