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FITC-LPSs from E. coli serotypes O111:B4 and O55:B5, diluted at 5 μg/ml in PBS-EDTA (pH 7.2) and acetate buffer-EDTA (pH 5.6) prepared 0.15 M, or the same buffers containing 1M NaCl, were added to hCAS-coated plates.
