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. 2016 Jun 1;11(6):e0156530. doi: 10.1371/journal.pone.0156530

Fig 6. Competitive hCAS-ELISA performed under acidic conditions.

Fig 6

FITC-LPSs (5 μg/ml) from E. coli serotypes O111:B4 (A) and O55:B5 (B) were preincubated with several concentrations of proteins and peptides (40, 10, 1 and 0.25 μg/ml) in acetate buffer-EDTA (pH 5.6) and added to casein hydrolysate (hCAS)-coated wells. Data are expressed as percentage inhibition of LPS binding to hCAS by the target molecule. Results are the means ±SD of duplicate wells. The average values of the optical density (492 nm) of control wells (without inhibitor) were 1.009 (±0.030, red dashed line) and 0.746 (±0.046, red dashed line) for serotypes O111:B4 and O55:B5, respectively. Differences were considered significant at p <0.05. NS: not significant. BSA, bovine serum albumin; HB, hemoglobin; HF1, histone f1 fraction; LF, lactoferrin; LSZ, lysozyme; MEL, melittin; MF6p, synthetic FhHDM-1/MF6p; MYO, myoglobin; P3L, A. simplex peptide: MCQCVQKYGTEFCKKRLA; PMX, polymyxin B.