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Rescue experiments of WT and TRPM4 variants. A, Western blot and quantification showing expression of WT and TRPM4 variants at the total and surface levels at 37°C and 28°C. Quantification was done on 3 different Western blots. B, Current density of WT and A432T at 37°C and 28°C recorded as the plateau phase. *P<0.05, **P<0.01 (WT at 28°C, n=4; WT at 37°C, n=6; A432T at 28°C, n=6; and A432T at 37°C, n=6). Temp indicates temperature; WT, wild type.
