Table 6.
Adventitia Aortic Wall Tissue Protein Biomarkers
| Control Adventitia, n=12 | AAA Adventitia, n=12 | P Value | |
|---|---|---|---|
| IL‐1b | 356 (196–492) | 939 (634–1283) | 0.0084 |
| IL1‐Ra | 7643 (3841–10 426) | 9441 (5647–10 677) | 0.3559 |
| IL‐4 | 283 (106–443) | 290 (122–446) | 0.8432 |
| IL‐5 | 2119 (1186–2690) | 3153 (2040–3450) | 0.0848 |
| IL‐6 | 3465 (1635–5930) | 9878 (5662–15 003) | 0.0290 |
| IL‐7 | 1599 (685–2128) | 1930 (1464–2179) | 0.3088 |
| IL‐8/CXCL8 | 2213 (1731–3794) | 5104 (2175–6823) | 0.1879 |
| IL‐9 | 3208 (2313–3707) | 3017 (2469–3626) | 0.9439 |
| IL‐10 | 1307 (1073–1556) | 1479 (1266–1997) | 0.1213 |
| IL‐12p70 | 1847 (180–2670) | 2534 (2080–3460) | 0.0671 |
| IL‐13 | 1082 (864–1550) | 1121 (970–1595) | 0.5383 |
| IL‐15b | 470 (0–916) | 533 (0–983) | 0.8951 |
| IL‐17A | 3334 (1970–4056) | 2879 (1668–3528) | 0.4237 |
| bFGF | 43 819 (39 739–48 884) | 32 588 (26 826–35 877) | 0.0014a |
| VEGF | 4082 (2898–5001) | 4493 (3010–4953) | 0.7728 |
| Eotaxin | 1680 (1182–1869) | 3074 (2777–4402) | 0.0004a |
| G‐CSF | 493 (480–3362) | 3903 (2406–4547) | 0.0346 |
| GM‐CSF | 3962 (3445–4358) | 4066 (3823–4337) | 0.7416 |
| IFN‐γ | 1443 (1017–3482) | 3222 (1935–4537) | 0.1131 |
| IP‐10/CXCL10 | 14 629 (13 125–16 341) | 16 686 (13 244–29 077) | 0.2786 |
| MCP‐1/CCL2 | 4590 (2542–5626) | 9095 (6280–11 674) | 0.0115 |
| MIP‐1a/CCL3 | 65 (50–133) | 248 (158–356) | 0.0116 |
| MIP‐1b/CCL4 | 1815 (1511–1999) | 5076 (4000–6878) | 0.0014a |
| PDGFβ | 6514 (5429–7793) | 6297 (5552–7633) | 0.8951 |
| RANTES/CCL5 | 11 149 (10 100–14 543) | 22 021 (16 661–24 664) | 0.0003a |
| TNF‐αb | 1617 (0–2486) | 1821 (0–3169) | 0.9461 |
Microdissected adventitia aortic wall tissue biomarkers. The greatest case/control differences were observed in this layer of the wall, in which eotaxin, MIP‐1b, and RANTES were significantly elevated, and bFGF was significantly decreased, in AAA. AAA indicates abdominal aortic aneurysm; bFGF, basic fibroblast growth factor; CCL, chemokine (C‐C motif) ligand; CXCL, chemokine (C‐X‐C motif) ligand; G‐CSF, granulocyte colony‐stimulating factor; GM‐CSF, granulocyte macrophage colony‐stimulating factor; IFN‐γ, interferon‐gamma; IL, interleukin; IP‐10, interferon‐gamma‐induced protein 10; MCP‐1, monocyte chemoattractant protein 1; MIP, macrophage inflammatory protein; PDGFβ, platelet‐derived growth factor beta; RANTES, regulated on activation, normal T‐cell expressed and secreted; TNF‐α, tumor necrosis factor alpha; VEGF, vascular endothelial growth factor.
Markers below the multiple testing threshold (P<0.0019).
Some samples recorded IL‐15 values below the detectable range of this assay and were assigned a rank order of 0. All inflammatory cytokines are measured in pg/100 mg tissue.