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. 2016 Apr 27;5(5):e003429. doi: 10.1161/JAHA.116.003429

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© 2016 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Gating strategy in heart tissue flow cytometry analysis. Cardiac graft tissue was stained for CD45 and Ly6G to quantify infiltration of leukocytes (CD45⁺ cells) or neutrophils (Ly6G⁺ cells), respectively.