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. 2016 Apr 27;5(5):e003429. doi: 10.1161/JAHA.116.003429

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© 2016 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Influence of RNase A on mixed lymphocyte reaction. A, CD4⁺ Teff cells were isolated from naive human PBMCs and exposed to buffer only or towards polyclonal antiCD3/antiCD28 antibodies; proliferation of resting Teff cells was carried out by a CFSE dilution assay. B, The proliferation of allogeneic stimulated Teff cells was analyzed in MLR (co‐culture of isolated Teff cells with donor PBMCs in a ratio of 1:4) in the absence or presence of RNase A. Data represent the analysis of 2 independent experiments (n=6) and are shown as means±SD. CFSE indicates carboxyfluorescein succinimidyl ester; MLR, mixed lymphocyte reaction; PBMC, peripheral blood mononuclear cells; Teff, effector T cell.