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. 2016 Mar 3;15(8):1099–1107. doi: 10.1080/15384101.2016.1156276

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Figure 4. — Immunofluorescent staining for Parkin after fractionated radiation (FR). (A) IF localization of Parkin in unirradiated TIG-3 and MRC-5 cells (0FR) and cells irradiated for 31 d (31FR). DNA was stained with Hoechst. (B) Images of Parkin staining in 0FR and 31FR ataxia telangiectasia mutated-deficient cells and complemented cells with and without N-acetyl-L-cysteine (NAC) treatment. (C) Percentage of Parkin-positive cells in indicated cells exposed to 0.01- or 0.05-Gy fractions and exposed to FR plus NAC. Asterisks indicate a significant increase or decrease in the percentage of Parkin-positive cells compared with control 0FR cells.