Figure 1.

PrimPol^−/− cells show decreased UV-C sensitivity with dose compared to wild type and Pol η^−/− in viability but not clonal survival assays. (A) Cell viability was measured after increasing doses of UV-C (48 hrs after damage) using Cell Titer Blue, lines represent an average of 3 repeats. (B) Cells were grown in the presence of increasing doses of 4NQO or media alone for 48 hrs followed by viability analysis with Cell Titer Blue, or after 48 hrs they were washed with PBS and grown for a further 72 hrs in media alone before viability analysis (C). This method was compared with clonal cell survival after UV-C, where cells were plated singularly after increasing doses of UV-C irradiation and growth to form a colony was counted (n = 3) (D). Significance was calculated using a students T-test, at 2 J/m² p < 0.05 WT: PrimPol^−/− cl1, WT: Pol η^−/− and PrimPol^−/− cl2 p < 0.01. Error bars represent standard deviation of repeat experiments in all cases.