Figure 8.

Participation of histone deacetylases in AA- or OA-induced global DNA methylation changes. A, DNA methylation response in MEFs genetically null for SIRT1, −2, or −6 (indicated as “null”) and matched WT controls, stimulated with 100 μM AA or OA for 24 h, or unstimulated (U). Statistical significance is in comparison with corresponding WT cells. B, Effects of inhibitors of SIRT1 (sirtinol, splitomycin) and broad range histone deacetylases (TSA) on the 100 μM AA-induced response. For statistics symbols and test, see legend of Fig. 1.