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Skp2 and Skp2B promote breast cancer cell proliferation and inhibit cell apoptosis. (A, B) Cell viability was measured by MTT assay 24, 36, 48, or 60 h following transfection of plasmids encoding Skp2 or Skp2B into MCF-7 and T47D cell lines. (C, D) Apoptosis was detected by flow cytometry analysis following transfection of plasmids encoding Skp2 or Skp2B into MCF-7 and T47D cell lines. Data in are the mean of 3 independent experiments. *P < 0.05, as compared with untreated cells.
