Figure 2.

Nutlin-3 induced p53 downregulated cMyc expression in activated T cells. (A) 10⁵ T cells were activated with PHA (1 µg/ml) and cultured without and with nutlin-3 (5 µM). Activated T cells were pulsed with ³H-thymidine at the indicated time point for 16 h (black bars - activated T cells cultured with the solvent control DMSO; gray bars – activated T cells cultured with nutlin-3). (B) cMyc, p53, p21 and p14ARF were detected at the indicated time points in T cells cultured with and without PHA (1 µg/ml) and with and without nutlin-3 (5 µM). (C) mRNA expression of cMyc, p21 and p53 measured by real time RT-PCR in T cells activated with PHA for 24 h and treated with or without nutlin-3 (5 µM) for the last 8 h (black bars - activated T cells cultured with the solvent control DMSO; gray bars - activated T cells cultured with nutlin-3). Values represent average of 3 experiments with SD.