Figure 1.

Schematic representation of the experimental procedure. Lentiviral vectors carrying reprogramming factors were introduced into the hESM01 cell line, and the stable clones were selected for further analysis (zero transgene expression, genome stability, in vitro and in vivo pluripotency). The resulting hESM01-OSKMN-DOX-n5 cell line was differentiated into 3 types of somatic cells. Magnetically separated cells were reprogrammed by DOX induction and iPSC clones generated from each cell type were chosen for genome-wide analysis of reprogramming traces, somatic memory, and iPSC specific markers using transcription and DNA methylation data.