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. 2016 Feb 26;15(7):986–997. doi: 10.1080/15384101.2016.1152425

Figure 2.

Figure 2.

Characterization of n5 somatic derivatives. (A) Morphology (phase contrast microscopy) and immunocytochemistry of fibroblast-like cells (F), neurons (N), and retinal pigment epithelial cells (R). TUBb3, β-tubulin (III); CHAT, choline acetyltransferase; CK, cytokeratin; RPE65, retinal pigment epithelium-specific 65 kDa protein. Signals corresponding to the respective markers are shown in red, with blue indicating nuclei stained with DAPI. Scale bar, 100 mM. (B) FACS analysis of TRA-1-60 expression in differentiated cells. Dotted lines represent isotype controls and antibody staining is shown in green. (C) FACS analysis of the purity of magnetically separated F, N, and R cells for corresponding somatic markers.