Figure 5.

The partial G1-S block induced by PP4 deficiency is associated with enhanced expression of CDK inhibitors p16/Ink4a and p15/Ink4b. (A, D-F) RNA was collected from T cells activated as in Fig. 3A at the specified time for qPCR analyses. Fold inductions relative to unstimulated WT T cells, after compensating for signals from β–actin, are shown for the respective genes (n = 3–7). (B-C) MACS-purified T cells were activated as above with their lysates collected at the indicated time for western blot analyses. Representative protein gel blotting results are shown (n = 3–5). 3 + 28, anti-CD3ϵ+anti-CD28 stimulation.