Figure 3.
Sertoli and Leydig cell development is unaltered in Ccnd1 deficient gonads through the mitotic:meiotic switch. Ccnd1 deficient somatic cells have unaltered expression of (A & B) pre-Sertoli and (C) developing Leydig cell markers. Oct4::GFP-negative somatic cells were isolated by FACS from E14.5, E15.5 M19-Ccnd1+/+ (M19-Ccnd1 WT) and M19-Ccnd1KO/KO (M19-Ccnd1 KO) gonads, and gene expression was analyzed by qPCR (n = 3-5). Gene expression at all embryonic time points is plotted relative to expression in E14.5 M19-Ccnd1 WT male somatic cells.