Figure 1.

Ribosomal uL11 protein is indispensable for full translational capacity. (A) translational fitness determination by the radiolabeled ³⁵S-methionine incorporation measurement. The results are an average of 3 independent experiments; ▪ - wild type stain, ▴ - ΔuL11AB mutant yeast strain, ♦ - complementation with the uL11 protein. Inset, the slope value determined for individual yeast strains, with the translation inhibition, which was quantified as a percent of translational impairment in respect to wild type cells. (B) ribosomal half-transit time determination. The incorporation of ³⁵S-methionine into total proteins (nascent and completed - diamonds) and completed (squares) are shown for each strain. The average half-transit time was determined by linear regression analysis as displacement between 2 lines and presented on the graph as inset - T 1/2. The radioactivity at each time point is presented as a mean of 3 independent measurements.