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Obtaining constructs expressing shRNA specific for CDH1. (A) The sequences of 21 nucleotide regions corresponding to the human CDH1 mRNA (Sequence ID: ref NM_004360.3), validated as siRNA #4 and #5 (bold) – most effective targets. (B) Effect of transduction of lentiviral constructs pLKO.1-shCDH1 on expression of E-cadherin in A549. RT-PCR analysis of CDH1 expression with corresponding shRNAs (#1–5). α-tubulin mRNA was analyzed as loading control.
