Table 1A.
DAVID functional annotation and enrichment analysis results. DAVID clustering analysis identified 15 significant functional groups ranging in enrichment from 4.03 to 1.65, using the “highest” stringency setting. Several of the highest ranking clusters are associated with cell activation, the expected physiological phenotype.
| Cluster | Description | Enrich-ment score | # genes | P-value range | Genes |
|---|---|---|---|---|---|
| Cluster 1 | positive regulation of leukocyte/lymphocyte/cell activation | 4.03 | 9 | 5.38E-5 – 1.4E-4 | CD38, IL2RA, CD80, CD3E, IL4R, IL13, RARA, ITPKB, TNFRSF4 |
| Cluster 2 | regulation of leukocyte/lymphocyte/cell activation | 3.4 | 10 | 1.97E-4 -6.77E-4 | CD38, IL2RA, CD80, ADORA2A, CD3E, IL4R, IL13, RARA, ITPKB, TNFRSF4 |
| Cluster 3 | regulation of leukocyte/lymphocyte/mononuclear cell proliferation | 2.63 | 6 | 9.84E-4 – 0.006 | CD38, IL2RA, CD80, CD3E, IL13, TNFRSF4 |
| Cluster 4 | tumor necrosis factor | 2.56 | 4 | 0.001 – 0.003 | TNF, TNFSF4, LTA, TNFSF8 |
| Cluster 5 | regulation of lymphocyte/Tcell differentiation | 2.55 | 5 | 0.001 – 0.011 | IL2RA, CD80, IL4R, RARA, ITPKB |
| Cluster 6 | leukocyte/lymphocyte/mononuclear cell proliferation | 2.55 | 5 | 0.002– 0.003 | SHB, ZBTB32, TNFSF4, CCND3, TNFRSF4 |
| Cluster 7 | phosphorylation-associated | 2.42 | 7 | 0.001 – 0.043 | CSF2, CARD14, TNF, CCND3, CD80, CD3E, CD81 |
| Cluster 8 | protein transport and localization | 2.15 | 7 | 0.005 – 0.012 | TNF, TNFSF4, NFKBIE, ADORA2A, IL13, TNFRSF4, LTA |
| Cluster 9 | T-helper/CD4 α β T cell differentiation | 2.12 | 3 | 0.003 – 0.017 | CD80, IL4R, RARA |
| Cluster 10 | C2 membrane targeting protein | 2.11 | 7 | 0.002 – 0.018 | PLCL2, HECW2, RGS3, SYT16, TC2N, RASA3, PRKCB |
| Cluster 11 | regulation of immunoglobulin/cytokine/protein secretion | 2.01 | 3 | 0.006 – 0.020 | TNF, TNFSF4, TNFRSF4 |
| Cluster 12 | regulation of phosphorylation/phosphorous metabolic process | 1.84 | 14 | 0.012 – 0.016 | CSF2, TNF, ADORA2A, CD3E, CCDC88C, NPRL2, TNFRSF4, CISH, CARD14, CD80, CCND3, RGS3, CD81, ZFYVE28 |
| Cluster 13 | cell death/apoptosis | 1.8 | 13 | 0.015 – 0.016 | CD38, PRUNE2, TNFRSF9, AHRR, TNF, IL2RA, ADORA2A, CD3E, MMP9, LGALS12, ARHGEF12, PDCD6, LTA, TNFSF8 |
| Cluster 14 | SANT family (DNA binding) | 1.78 | 4 | 0.005–0.038 | CRAMP1L, TRERF1, RERE, NCOR2 |
| Cluster 15 | calcium/metal ion homeostasis | 1.65 | 8 | 0.013 – 0.046 | S1PR3, CD38, CD55, CCR5, CCR1, TPT1, LOC389787, LOC285741, CCL5, PRKCB |