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. 2016 Apr 20;15(8):1144–1155. doi: 10.1080/15384101.2016.1158376

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© 2016 Taylor & Francis

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Figure 5. — Effects of the chorion-derived stem cell conditioned medium (CDSC-CNM) on photo-aged HaCaT cell migration. Images were acquired at 0 and 16 h after incubation using fluorescence or a phase-contrast microscope in an in vitro scratch assay. (A) HaCaT cells were cultured with different concentrations of CDSC-CNM. The dotted lines define the areas of cells migration. The non-irradiated HaCaT cells closed the scratch at 16 h (photo not shown). (B) The rate of migration was measured by quantifying the total distance that the HaCaT cells had moved relative to the scratch (marked by imaginary dotted lines). The data are expressed as the mean of 6-8 replicates ± SD.* (P < 0.05) and *** (P < 0.001) indicate significant differences compared with non-irradiated cells in the same concentrations of CDSC-CNM.