Figure 1.
OGA expression is reduced in cell lines 040 and 877. (A) Stable shRNA mediated OGA knockdown HeLa cells lines were established with a significant decrease in OGA protein expression as judged by western blot and quantified by densitometry. OGT protein expression was not significantly changed while total O-GlcNAc levels did increased slightly. (B) OGA mRNA is lower in both the 040 and 877 knockdown lines. C, OGA enzymatic activity was significantly decreased in the stable OGA KD lines compared to the control cells. All experiments were performed with at least 3 biological replicates (* indicates P < 0.05, Student t-test).