Figure 7.
OGA 877 KD cells have reduced histone H3 phosphorylation. Cells were synchronized into M phase by double thymidine block and release (A), prophase by nocodazole treatment (B), or metaphase-anaphase with STC. (C) Histones were purified by acid extraction and phosphorylation of histone H3 Serine 10 and Threonine 3 was measured. H3 T3 phosphorylation was lower in the double thymidine block and released 877 cells. Graphs of normalized densitometric analysis for phosphorylation of S10H3 or T3H3 to total histone H3 (n = 3; *, p < 0.05 versus control). Data are expressed as means ± SE. All experiments were performed with at least 3 biological replicates.