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NuMA and EWS O-GlcNAcylation increases in OGA knockdown cells. (A-B) NuMA and EWS was precipitated from prophase extracts using specific antibodies to NuMA or EWS. Blots were then probed for O-GlcNAc using the RL-2 O-GlcNAc antibody. Both NuMA and EWS O-GlcNAcylation were increased in 040 and 877 OGA knockdown cells. All experiments were performed with at least 3 biological replicates.
