Table 1.
Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) species changed to a statistically significant extent 24 h post-IR-induced AKI
| Lipid | MS/MS, m/z | Intensity Change | Fold- Change | Lipid | MS/MS, m/z | Intensity Change | Fold- Change |
|---|---|---|---|---|---|---|---|
| PC 34:1 | 760.6_184.1 | 659.1→1295.1 | +2 | PE 34:1 | 718.6_198.1 | 62.3→1.8 | −34.6 |
| PC 34:4 | 754.6_184.1 | 1082.2→2,014.7 | +1.9 | PE 36:0 | 748.6_198.1 | 9900.4→155.8 | −63.6 |
| PC 34:6 | 750.6_184.1 | 940.4→244.1 | −3.9 | PE 36:1 | 746.6_198.1 | 3229.9→31.6 | −102.2 |
| PC 36:0 | 790.6_184.1 | 418.1→1290.5 | +3.1 | PE 38:0 | 776.6_198.1 | 1057.6→14.9 | −71.0 |
| PC 36:1 | 788.7_184.1 | 398.6→1461.5 | +3.7 | PE 38:1 | 774.6_198.1 | 267.5→1.5 | −178.3 |
| PC 36:2 | 786.6_184.1 | 927.0→2635.3 | +2.8 | PE 38:4 | 768.7_198.1 | 273.8→11.6 | −23.6 |
| PC 36:3 | 784.6_184.1 | 1584.5→3037.3 | +1.9 | PE 38:6 | 764.6_198.1 | 81.1→ND | − |
| PC 36:4 | 782.6_184.1 | 698.4→1290.8 | +1.9 | PE 40:0 | 804.7_198.1 | 189.4→3.6 | −52.6 |
| PC 38:2 | 814.6_184.1 | 3757.9→6117.2 | +1.6 | PE 40:1 | 802.7_198.1 | 723.0→1.0 | −723 |
| PC 38:4 | 810.7_184.1 | 4265.8→7589.2 | +1.8 | PE 40:2 | 800.7_198.1 | 1089.5→3.4 | −320.4 |
| PC 38:5 | 808.7_184.1 | 1265.6→2983.7 | +2.4 | PE 40:3 | 798.7_198.1 | 304.8→11.0 | −27.7 |
| PC 38:6 | 806.7_184.1 | 877.4→1505.9 | +1.7 | PE 42:0 | 832.7_198.1 | 190.4→3.8 | −50.1 |
| PC 40:2 | 842.7_184.1 | 1641.7→2940.4 | +1.8 | PE 42:3 | 826.7_198.1 | 2551.5→55.9 | −45.6 |
| PC 40:4 | 838.7_184.1 | 1623.9→3683.5 | +2.3 | PE 42:4 | 824.7_198.1 | 999.9→30.6 | −32.7 |
| PC 42:4 | 866.7_184.1 | 1335.2→2610.5 | +2.0 | PE 42:5 | 822.7_198.1 | 245.0→14.8 | −16.6 |
| PC 42:5 | 864.7_184.1 | 3081.6→5870.6 | +1.9 | PE 42:6 | 820.7_198.1 | 177.8→11.9 | −14.9 |
| PC 42:6 | 862.7_184.1 | 5128.4→7823.7 | +1.5 | PE 44:0 | 860.7_198.1 | 307.7→22.4 | −13.7 |
| PC 44:1 | 900.7_184.1 | 1402.1→2530.1 | +1.8 | PE 44:1 | 858.7_198.1 | 340.5→47.2 | −7.2 |
| PC 44:6 | 890.7_184.1 | 4260.8→6964.1 | +1.6 | PE 44:2 | 856.7_198.1 | 435.2→28.2 | −15.4 |
| PC 46:1 | 928.8_184.1 | 1072.0→1759.3 | +1.6 | PE 44:3 | 854.7_198.1 | 763.8→18.8 | −40.6 |
| PC 48:5 | 948.8_184.1 | 1184.5→1913.6 | +1.6 | PE 44:4 | 852.7_198.1 | 438.6→15.8 | −27.8 |
| PC 48:6 | 946.8_184.1 | 1154.4→1793.8 | +1.6 | PE 44:5 | 850.7_198.1 | 196.4→5.1 | −38.5 |
| PE 34:0 | 720.6_198.1 | 285.0→4.1 | −69.5 | PE 44:6 | 848.7_198.1 | 201.1→7.2 | −27.9 |
| PE 46:0 | 888.7_198.1 | 810.2→28.4 | −28.5 | PE 48:0 | 916.8_198.1 | 612.8→28.4 | −21.6 |
| PE 46:2 | 884.7_198.1 | 526.6→17.9 | −29.4 | PE 48:1 | 914.8_198.1 | 445.7→14.5 | −30.7 |
| PE 46:3 | 882.8_198.1 | 253.2→6.7 | −37.8 | PE 48:2 | 912.8_198.1 | 802.8→14.2 | −56.5 |
| PE 46:4 | 880.8_198.1 | 422.0→10.6 | −39.8 | PE 48:3 | 910.7_198.1 | 447.0→15.9 | −28.1 |
| PE 46:5 | 878.8_198.1 | 862.7→29.0 | −29.8 | PE 48:5 | 906.8_198.1 | 764.7→21.5 | −35.6 |
| PE 46:6 | 876.7_198.1 | 1408.2→54.5 | −25.8 | PE 48:6 | 904.8_198.1 | 1069.4→38.8 | −27.6 |
IR, ischemia-reperfusion; AKI, acute kidney injury; tandem mass spectrometry (MS/MS), m/z: parent ion m/z followed by fragment ion m/z used for lipid identification; intensity change, average lipid intensity sham→average lipid intensity IR. Reductions are highlighted in bold. Note that all statistically significant changes in PEs 24 h post-IR were reductions.