Table 5.
Glycosphingolipid species changed to a statistically significant extent 24 h post-IR-induced AKI
| Lipid | MS/MS, m/z | Intensity Change | Fold-Change |
|---|---|---|---|
| Hex3Cer 26:3;3 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 924.8_262.2 | ND→18.7 | + |
| Hex3Cer 26:4;3 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 922.8_262.2 | 1.7→16.2 | +9.5 |
| Hex3Cer 27:3;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 954.8_262.2 | ND→12.3 | + |
| Hex3Cer 27:4;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 952.8_262.2 | ND→13.5 | + |
| Hex3Cer 28:0;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 974.8_262.2 | 2.5→19.7 | +7.9 |
| Hex3Cer 28:1;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 972.8_262.2 | 0.8→17.5 | +21.9 |
| Hex3Cer 29:2;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 984.8_264.2 | 3.7→16.2 | +4.4 |
| Hex3Cer 30:0;3 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 986.8_264.2 | 4.6→13.6 | +3.0 |
| Hex3Cer 30:1;3 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 984.8_262.2 | 3.1→13.8 | +4.5 |
| Hex3Cer 30:4;3 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 978.8_264.2 | 1.6→39.2 | +24.5 |
| Hex3Cer 30:4;3 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 978.8_262.2 | 2.3→41.3 | +18.0 |
| Hex3Cer 32:0;3 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 1014.8_264.2 | 34.8→17.7 | −2.0 |
| Hex3Cer 32:4;3 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 1006.9_262.2 | 1.9→32.1 | +16.9 |
| Hex3Cer 32:4;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 1022.9_262.2 | 1.1→15.7 | +14.3 |
| Hex3Cer 34:2;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 1054.9_262.2 | 0.3→17.6 | +58.7 |
| Hex3Cer 38:1;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 1113.0_264.2 | 14.5→4.4 | −3.3 |
| Hex3Cer 38:2;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 1111.0_264.2 | 21.8→12.4 | −1.8 |
| Hex3Cer 38:4;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 1107.0_264.2 | 20.6→6.6 | −3.1 |
| Hex3Cer 39:3;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 1122.9_264.2 | 18.1→2.4 | −7.5 |
| Hex3Cer 40:1;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 1141.0_264.2 | 20.7→5.6 | −3.7 |
| Hex2Cer 26:0;3 (LCB 18:0;2-2H2O) | 768.7_266.2 | ND→19.6 | + |
| Hex2Cer 26:3;2 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 746.6_262.3 | 1.0→18.7 | +18.7 |
| Hex2Cer 30:3;2 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 802.7_262.3 | 3.3→74.5 | +22.6 |
| Hex2Cer 30:3;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 834.7_262.3 | 20.3→8.6 | −2.4 |
| Hex2Cer 32:3;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 862.7_262.3 | 18.4→1.4 | −13.1 |
| Hex2Cer 32:3;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 862.7_264.3 | ND→19.0 | +19 |
| Hex2Cer 34:1;3 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 878.8_262.3 | ND→16.2 | +16.2 |
| Hex2Cer 34:1;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 894.7_262.3 | ND→17.6 | +17.6 |
| Hex2Cer 36:3;3 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 894.7_262.3 | ND→17.6 | +17.6 |
| Hex2Cer 36:3;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 918.8_264.2 | 18.4→9.0 | −2.0 |
| Hex2Cer 39:4;2 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 926.8_262.2 | ND→16.5 | + |
| Hex2Cer 40:2;3 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 960.8_262.2 | 1.0→19.7 | +19.7 |
| Hex2Cer 40:3;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 974.8_262.2 | 0.9→31.1 | +34.6 |
| Hex2Cer 41:1;3 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 976.8_264.2 | 9.2→39.4 | +4.3 |
| HexCer 33:2;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 716.6_264.3 | ND→13.5 | + |
| Cer 28:1;2 (LCB 18:1;2-H2O, LCB 18:0;3-2H2O) | 454.3_282.3 | ND→18.5 | + |
| CerP 26:1;4 (LCB 18:1;2-H2O, LCB 18:0;3-3H2O) | 538.4_264.3 | ND→200.7 | + |
| CerP 44:1;3 (LCB 18:2;2-H2O, LCB 18:1;3-3H2O) | 774.6_262.3 | 1.4→25.1 | +17.9 |
| CerPE 36:3;4 (LCB 18:2;2-H2O, LCB 18:1;3-3H2O) | 717.6_262.3 | 2.7→29.1 | +10.8 |
| CerPE 38:1;3 (LCB 18:2;2-H2O, LCB 18:1;3-3H2O) | 733.6_262.3 | ND→11.7 | + |
| SGalCer 26:0;3 (LCB 18:0;2-2H2O) | 686.5_266.3 | 353.3→6.3 | −56.1 |
| SGalCer 26:1;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 700.5_264.3 | ND→58.2 | + |
| SGalCer 29:3;4 (LCB 18:1;2-2H2O, LCB 18:0;3-2H2O) | 738.6_282.3 | ND→15.8 | + |
| SGalCer 29:4;3 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 720.6_264.2 | 4.0→24.3 | +6.1 |
| SGalCer 30:3;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 752.6_262.3 | 17.5→ND | − |
| SGalCer 30:4;2 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 718.6_262.3 | 24.5→349.3 | +14.3 |
| SGalCer 30:4;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 750.6_262.3 | 71.1→2.2 | +32.3 |
| SGalCer 31:1;4 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 770.6_262.3 | 14.2→ND | − |
| SGalCer 32:0;3 (LCB 18:0;2-2H2O) | 770.6_266.3 | 130.8→2.0 | −65.4 |
| SGalCer 32:0;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 786.6_282.3 | 26.0→1.2 | −21.7 |
| SGalCer 34:0;3 (LCB 18:0;2-2H2O) | 798.6_266.3 | 79.4→ND | − |
| SGalCer 34:0;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 814.7_282.3 | 31.7→ND | − |
| SGalCer 38:4;2 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 830.7_262.3 | 2.2→100.2 | +45.6 |
| SGalCer 40:0;4 (LCB 18:1;2-2H2O, LCB 18:0;3-3H2O) | 898.8_264.2 | 6.0→26.4 | +4.4 |
| SGalCer 40:1;3 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 880.8_262.3 | ND→23.6 | + |
| SGalCer 44:4;2 (LCB 18:2;2-2H2O, LCB 18:1;3-3H2O) | 914.8_262.2 | 2.9→19.2 | +6.6 |
Cer, ceramide; Hex3Cer, trihexosylceramide; Hex2Cer, dihexosylceramide; CerP, ceramide 1-phosphate; CerPE, ceramide phosphoethanolamine; SGalCer, 3-O-sulfo galactosylceramide; LCB, long-chain base. Text in parentheses shows possible LCBs based on fragment ion m/z. Reductions are highlighted in bold.