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Assessment of fluorescent dye albumin conjugates on FcRn binding using MST analysis
| Fluorophore Conjugate (Ratio of Dye to Albumin) | pH 6 KD, μM) | pH 7.4 KD, μM |
|---|---|---|
| CF-594 maleimide* | ||
| 1:3 | 1.5 | NB |
| Texas red-X* | ||
| 1:1 | 5.4 | NB |
| Texas red* | ||
| 4:1 | >40 | NB |
| Alexa 488* | ||
| 4.5:1 | NB | NB |
| Fluorescein | ||
| 6.4:1 | 69 | 1.0 |
| Alexa 568 | ||
| 4:1 | 17 | NB |
| Texas red-X | ||
| 2:1 | 10 | NB |
| Texas red-X | ||
| 5:1 | >100 | NB |
| Texas red | ||
| 1:1 | 6 | 11 |
| Alexa 647 maleimide | ||
| 1:1 | 11 | NB |
| DQ BSA | NB | NB |
| No dye | 11.6 | >100 |
Data from present study.
