Video 5. Vacuoles in Fig4^-/- OLs appear and disappear within hours.

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DOI: 10.7554/eLife.13023.020

Time-lapse live cell analysis of Fig4^-/- primary OPC/OLs imaged with an IncuCyte ZOOM microscope. Phase contrast images were taken every 2 hr over a time interval of 60 hr. The majority of Fig4^-/- cells contain large perinuclear vacuoles. Some of these vacuoles appear and disappear within hours (n = 3). Scale bar = 60 µm.

DOI: http://dx.doi.org/10.7554/eLife.13023.020