Video 3. 3D reconstruction of mitochondria and vacuoles in old dnm1△ cells.
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DOI: 10.7554/eLife.13943.006
A representative 3D reconstruction of an old dnm1△ cell with the same characteristics as those depicted in Figure 1B showing the presence of mitochondria (green, marked with Tom70-GFP), but the absence of small mitochondrial vesicle-like fragments outside of the severely fragmented vacuole (red, marked with Vph1-mCherry). To permit visualization of the vacuole lumen, the vacuole isosurface rendering becomes 60% transparent in the middle of the movie. Budscars (blue, calcofluor) at the beginning of the movie indicate the cell’s old age.