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. 2016 Jun 1;5:e13325. doi: 10.7554/eLife.13325

Figure 1. The aux/iaaΔ mutant displays a severe phenotype.

(A) Scheme representing the auxin-signaling pathway in WT plants (left panel) and in the aux/iaaΔ mutant (right panel). (B) GUS expression in PIAA1B:GUS and aux/iaaΔ plants carrying the PIAA1B:GUS reporter. Arrows denote GUS expression. (C) WT and the aux/iaaΔ mutant grown for one month on BCD medium, stimulating gametophore development without auxin or with different concentrations of 1-naphthalene-acetic acid (NAA). (D) Microscopic enlargement of WT gametophores: left panel- leafy gametophores grown without exogenous auxin. Right panel-ectopic rhizoids emerging from a gametophore grown on 12.5 μM NAA. (E–H) Plants grown for one month on BCDAT medium to promote filamentous growth. (E, F) WT plant grown without auxin or with 12.5 μM NAA, respectively. (G) iaa2-P328S degron-motif mutant. (H) The aux/iaaΔ mutant has a variable phenotype. (I) WT and aux/iaaΔ mutant grown for one month on BCDAT or BCDAT supplemented with 10 μM L-Kyn. Scale bars: 1 mm (B), 0.5 cm (C, H, I), the scale bar in H also corresponds to E–G, 0.5 mm (D).

DOI: http://dx.doi.org/10.7554/eLife.13325.003

Figure 1.

Figure 1—figure supplement 1. The aux/iaaΔ is a null mutant that displays constitutive auxin response.

Figure 1—figure supplement 1.

(A) PCR validating the absence of the genomic sequence of IAA1A, IAA1B, and IAA2 after CRE recombination. Primer pairs used: PML 749, 751 (IAA1A), PML 750, 751 (IAA1B), PML753, 754 (IAA2). (B) qPCR validating that IAA1A, IAA1B, and IAA2 are not expressed. The expression of the housekeeping gene EF1α is normal in the mutant and is used as a control.
DOI: http://dx.doi.org/10.7554/eLife.13325.004
Figure 1—figure supplement 2. Early stages of filamentous growth of WT and aux/iaaΔ mutant.

Figure 1—figure supplement 2.

(A) Six WT and aux/iaaΔ mutant plants were transferred to BCD medium four days after protoplast recovery and measured for 5 days. Error bars represent s.e.m. (B) Protonemal filaments of WT and aux/iaaΔ plants grown on BCD for two weeks following protoplast recovery without auxin or with 0.5 μm NAA. (C) Confocal images visualizing chloroplasts in WT and aux/iaaΔ plants seven days after protoplast recovery. Chloroplasts are visualized in white. Scale bars: 0.25 mm (B), 200 μm (C).
DOI: http://dx.doi.org/10.7554/eLife.13325.005