(A) Scheme representing the auxin-signaling pathway in WT plants (left panel) and in the aux/iaaΔ mutant (right panel). (B) GUS expression in PIAA1B:GUS and aux/iaaΔ plants carrying the PIAA1B:GUS reporter. Arrows denote GUS expression. (C) WT and the aux/iaaΔ mutant grown for one month on BCD medium, stimulating gametophore development without auxin or with different concentrations of 1-naphthalene-acetic acid (NAA). (D) Microscopic enlargement of WT gametophores: left panel- leafy gametophores grown without exogenous auxin. Right panel-ectopic rhizoids emerging from a gametophore grown on 12.5 μM NAA. (E–H) Plants grown for one month on BCDAT medium to promote filamentous growth. (E, F) WT plant grown without auxin or with 12.5 μM NAA, respectively. (G) iaa2-P328S degron-motif mutant. (H) The aux/iaaΔ mutant has a variable phenotype. (I) WT and aux/iaaΔ mutant grown for one month on BCDAT or BCDAT supplemented with 10 μM L-Kyn. Scale bars: 1 mm (B), 0.5 cm (C, H, I), the scale bar in H also corresponds to E–G, 0.5 mm (D).
DOI:
http://dx.doi.org/10.7554/eLife.13325.003