Genes | Primers | Cloning procedure | Backbone | |
---|---|---|---|---|
IAA1A | 5’ region | PML61, 62 | BamHI ligation | pBHRF2 |
3’ region | PML59, 60 | SpeI ligation | ||
IAA1B | 5’ region | PML52, 53 | BamHI ligation | pBNRF-GUS |
3’ region | PML59, 60 | SpeI ligation | ||
IAA2 | 5’ region | PML63, 64 | BamHI ligation | pBNRF |
3’ region | PML65, 66 | SpeI ligation | ||
ARFb4 | 5’ region | PML599, 600 | BamHI ligation | pBNRF-GUS/ pBNRF |
3’ region | PML601, 602 | SpeI ligation | ||
ARFb2 | 5’ region | PML677, 678 | BamHI ligation | pBHRF2 |
3’ region | PML679, 680 | SalI ligation |