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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Nat Protoc. 2010 Sep 2;5(9):1583–1595. doi: 10.1038/nprot.2010.117

SDS gel (12%) component ml

H2O 3.3
Acrylamide (30%) 4.0
Tris (pH 8.8; 1.5 M) 2.5
SDS (10%) 0.1
Ammonium persulfate (10%) 0.1
TEMED 0.004