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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Nature. 1996 May 30;381(6581):418–421. doi: 10.1038/381418a0

FIG. 2.

FIG. 2

Microscopic appearance of rat spermatogenesis in mouse seminiferous tubules. a, Seminiferous tubule of transgenic (MT-lacZ) rat. Sertoli cells along basement membrane are uniformly stained. All germ cell stages stain depending on substrate penetration. Centre tubule shows staining of pachytene spermatocytes. Stain, X-gal and neutral fast red (NFR). b, Seminiferous tubule of control rat. No cells are stained blue by X-gal. The long, thin heads of mature rat spermatozoa are evident. Stain, X-gal and NFR. c, Seminiferous tubule of nude mouse treated with busulphan. No germ-cell stages are present; only Sertoli cells can be seen on the basement membrane of the tubule. Stain, NFR. d, Seminiferous tubule of busulphan-treated recipient nude mouse showing rat spermatogenesis from transplanted cells (left testis from mouse 777). Rat pachytene spermatocytes and round spermatids are heavily stained blue. Sertoli cells (arrowheads) do not stain blue. Stain, X-gal and NFR. e, Seminiferous tubule from left testis of mouse 777. Rat preleptotene and pachytene spermatocytes are stained blue. Stain, X-gal plus periodic acid Schiff and haematoxylin, which emphasizes acrosome and head shape of rat spermatozoa. f, Seminiferous tubule from left testis of mouse 775. Rat preleptotene and pachytene spermatocytes are stained blue. Sertoli cells (arrowheads) do not stain blue. Stain, X-gal plus haematoxylin and eosin to emphasize normal morphology of spermatogenesis arising from transplanted rat stem cells. Scale bar, 50 μm.