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PilS R24E neither interacts with PilA E5K nor autoregulates pilA transcription in vivo. (A) The conserved R24 residue of PilS was substituted with a glutamic acid. When expressed from the chromosome, PilS R24E activated pilA transcription in the lux-pilA assay, but was no longer responsive to PilA overexpression. (B) In the T25-PilS fusion, the R24E PilS variant failed to interact with T18-WT PilA or T18-PilA E5K (gray bars).
