Fig. S7.

Western blots for the detection of CRY1 and CRY2 in Xenopus samples. Stage 51 (lane 1) and adult (lane 2) Xenopus protein samples were run on 4–12% polyacrylamide gels and transferred to nitrocellulose membrane for Western blotting to detect CRY1 or CRY2 using a commercially sourced antibody (Aviva Systems Biology; 1:5,000 for CRY1 and 1:1,000 for CRY2). Positive bands at ∼70 and 65 kDa were obtained for both samples. This result revealed that the antibody specifically detected proteins of the correct size for Xenopus cryptochromes.